Concentrations (mm) | |||||
---|---|---|---|---|---|
|
|||||
A | B | C | D | E | |
K-Asparate* [1.23] | 300 | 300 | 305 | 310 | 360 |
KCl | 65 | 65 | 65 | 65 | 10 |
K-Hepes (pH 7.3) | 150 | 150 | 150 | 150 | 150 |
Glucose | 11 | 11 | 11 | 11 | 11 |
K2-Glutathione | 10 | 10 | 10 | 10 | 10 |
CaCl2 | 0 | 7.5 | 25 | 35 | 45 |
K3-DPTA* [4.5] | 0 | 50 | 50 | 50 | 0 |
K2-EGTA* [∼7] | 50 | 0 | 0 | 0 | 0 |
BTC* | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
NPE* [∼7] (CaCl2) | 5(0) | 5(5) | 5(5) | 5(5) | 5(5) |
Measured [Ca2+] (μm) | |||||
---|---|---|---|---|---|
|
|||||
A | B | C | D | E | |
111 % solution | < 0.01 | 5.88 | 33.1 | 74.6 | 6440 |
Pre-flash | < 0.01 | 5.95 | 33.1 | 74.5 | 6320 |
Post-flash | < 0.01 | 7.52 | 37.9 | 87.4 | 6610 |
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Top part of table shows composition of calibration solutions A–E.
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↵* Ca2+-binding compound with pK values shown in square brackets. Bottom part of table shows measured [Ca2+] in the 111 % solution containing the first eight constituents and used to make final solutions, or calculated using pK values in final solutions before (Pre-flash) and after (Post-flash) single full strength (no neutral density filter) photolysis of NPE.